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Publications

  • Carlson J, Ahmed M, Hunter R, Hoque SF, Benoit JB, Chiurillo MA, Lander N. TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi. mBio. 2025 Jun 11;16(6):e0099425. doi: 10.1128/mbio.00994-25.

  • Bertolini MS, Cline SE, Chiurillo MA, Mantilla BS, Eidex A, Crowe LP, Qiu D, Jessen HJ, Saiardi A, Docampo R. Generation of inositol polyphosphates through a phospholipase C-independent pathway involving carbohydrate and sphingolipid metabolism in Trypanosoma cruzi. mBio. 2025, e0331824. doi: 10.1128/mbio.03318-24.

  • Chiurillo MA. mSphere of Influence: The challenge of screening for essential protein kinases in Trypanosoma cruzi. mSphere. 2025;10(2):e0059824. doi: 10.1128/msphere.00598-24.

  • Lander N, Chiurillo MA. A key regulator of male gametogenesis in Cryptosporidium. Trends Parasitol. 2024;40(8):662-663. doi: 10.1016/j.pt.2024.06.007.

  • Chiurillo MA, Carlson J, Bertolini MS, Raja A, Lander N. Gene editing of putative cAMP and Ca2+-regulated proteins using an efficient cloning-free CRISPR/Cas9 system in Trypanosoma cruzi. J Eukaryot Microbiol. 2023;70(6):e12999. doi: 10.1111/jeu.12999.

  • Chiurillo MA, Carlson J, Bertolini MS, Raja A, Lander N. Dual localization of receptor-type adenylate cyclases and cAMP response protein 3 unveils the presence of two putative signaling microdomains in Trypanosoma cruzi. mBio. 2023;e0106423. doi: 10.1128/mbio.01064-23.

  • Teixeira TL, Chiurillo MA, Lander N, Rodrigues CC, Onofre TS, Ferreira ÉR, Yonamine CM, Santos JG, Mortara RA, da Silva CV, da Silveira JF. Ablation of the P21 gene of Trypanosoma cruzi provides evidence of P21 as a mediator in the control of epimastigote and intracellular amastigote replication. Front Cell Infect Microbiol. 2022;12:799668. doi: 10.3389/fcimb.2022.799668

  • Chiurillo MA, Jensen BC, Docampo R. Drug Target Validation of the Protein Kinase AEK1, Essential for Proliferation, Host Cell Invasion, and Intracellular Replication of the Human Pathogen Trypanosoma cruzi. Microbiol Spectr. 2021 31;9(2):e0073821. doi: 10.1128/Spectrum.00738-21

  • Chiurillo MA, Lander N. The long and winding road of reverse genetics in Trypanosoma cruzi. Microb Cell. 2021;8(9):203-207. doi: 10.15698/mic2021.09.758

  • Docampo R, Vercesi AE, Huang G, Lander N, Chiurillo MA, Bertolini M. Mitochondrial Ca2+ homeostasis in trypanosomes. Int Rev Cell Mol Biol. 2021 362:261-289. doi: 10.1016/bs.ircmb.2021.01.002

  • Dos Santos GRR, Rezende Leite AC, Lander N, Chiurillo MA, Vercesi AE, Docampo R. Trypanosoma cruzi Letm1 is involved in mitochondrial Ca2+ transport, and is essential for replication, differentiation, and host cell invasion. FASEB J. 2021;35(7):e21685. doi: 10.1096/fj.202100120RR

  • Negreiros RS, Lander N, Chiurillo MA, Vercesi AE, Docampo R. Mitochondrial Pyruvate Carrier Subunits Are Essential for Pyruvate-Driven Respiration, Infectivity, and Intracellular Replication of Trypanosoma cruzi. mBio. 2021;12(2):e00540-21. doi: 10.1128/mBio.00540-21

  • Lander N, Chiurillo MA, Docampo R. Signaling pathways involved in environmental sensing in Trypanosoma cruzi. Mol Microbiol. 2021;115(5):819-828. doi: 10.1111/mmi.14621.

  • Chiurillo MA, Lander N, Vercesi AE, Docampo R. IP3 receptor-mediated Ca2+ release from acidocalcisomes regulates mitochondrial bioenergetics and prevents autophagy in Trypanosoma cruzi. Cell Calcium. 2020;92:102284. doi: 10.1016/j.ceca.2020.102284.

  • Faktorová D, Nisbet RER, Fernández Robledo JA, Casacuberta E, Sudek L, Allen AE, Ares M Jr, Aresté C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget FY, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, et al. Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nat Methods. 2020;17(5):481-494. doi: 10.1038/s41592-020-0796-x.

  • Lander N, Chiurillo MA, Docampo R. CRISPR/Cas9 Technology Applied to the Study of Proteins Involved in Calcium Signaling in Trypanosoma cruzi. Methods Mol Biol. 2020;2116:177-197. doi: 10.1007/978-1-0716-0294-2_13.

  • Lander N, Chiurillo MA. State-of-the-art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids. J Eukaryot Microbiol. 2019;66(6):981-991. doi: 10.1111/jeu.12747.

  • Chiurillo MA, Lander N, Bertolini MS, Vercesi AE, Docampo R. Functional analysis and importance for host cell infection of the Ca2+-conducting subunits of the mitochondrial calcium uniporter of Trypanosoma cruzi. Mol Biol Cell. 2019;30(14):1676-1690. doi: 10.1091/mbc.E19-03-0152.

  • Bertolini MS, Chiurillo MA, Lander N, Vercesi AE, Docampo R. MICU1 and MICU2 play an essential role in mitochondrial Ca2+ uptake, growth, and infectivity of the human pathogen Trypanosoma cruzi. mBio. 2019;10(3):e00348-19. doi: 10.1128/mBio.00348-19.

  • Lander N, Chiurillo MA, Docampo R. Genome editing by CRISPR/Cas9 in Trypanosoma cruzi. Methods Mol Biol. 2019;1955:61-76. doi: 10.1007/978-1-4939-9148-8_5.

  • Lander N, Chiurillo MA, Bertolini MS, Storey M, Vercesi AE, Docampo R. Calcium-sensitive pyruvate dehydrogenase phosphatase is required for energy metabolism, growth, differentiation, and infectivity of Trypanosoma cruzi. J Biol Chem. 2018;293(45):17402-17417. doi: 10.1074/jbc.RA118.004498.

  • Lander N, Chiurillo MA, Bertolini MS, Docampo R, Vercesi AE. The mitochondrial calcium uniporter complex in trypanosomes. Cell Biol Int. 2018;42(6):656-663. doi: 10.1002/cbin.10928.

  • Lander N, Chiurillo MA, Vercesi AE, Docampo R. Endogenous C-terminal tagging by CRISPR/Cas9 in Trypanosoma cruzi. Bio Protoc. 2017;7(10):e2299. doi: 10.21769/BioProtoc.2299.

  • Chiurillo MA, Lander N, Bertolini MS, Storey M, Vercesi AE, Docampo R. Different roles of mitochondrial calcium uniporter complex subunits in growth and infectivity of Trypanosoma cruzi. mBio. 2017;8(3):e00574-17. doi: 10.1128/mBio.00574-17.

  • Chiurillo MA, Antonio CR, Mendes Marini M, Torres de Souza R, Franco da Silveira J. Chromosomes ends and telomere biology of trypanosomatids. In eBook: “Molecular and Cellular Biology of Pathogenic Trypanosomatids”. eBook series “Frontiers in Parasitology”, Bentham Sciences Publishers LTD. 2017; pp. 104-133. doi: 10.2174/9781681084053117010006.

  • Lander N, Chiurillo MA, Storey M, Vercesi AE, Docampo R. CRISPR/Cas9-mediated endogenous C-terminal tagging of Trypanosoma cruzi genes reveals the acidocalcisome localization of the Inositol 1,4,5-Trisphosphate receptor. J Biol Chem. 2016;291(49):25505-25515. doi: 10.1074/jbc.M116.749655.

  • Lander N, Chiurillo MA, Docampo R. Genome editing by CRISPR/Cas9: A game change in the genetic amnipulation of protists. J Eukaryot Microbiol. 2016;63(5):679-90. doi: 10.1111/jeu.12338.

  • Chiurillo MA, Moraes Barros RR, Souza RT, Marini MM, Antonio CR, Cortez DR, Curto MÁ, Lorenzi HA, Schijman AG, Ramirez JL, da Silveira JF. Subtelomeric I-SceI-mediated double-Strand breaks are repaired by homologous recombination in Trypanosoma cruzi. Front Microbiol. 2016;7:2041. doi: 10.3389/fmicb.2016.02041.

  • Chiurillo MA, Cortez DR, Lima FM, Cortez C, Ramirez JL, Martins AG, Serrano MG, Teixeira MM, da Silveira JF. The diversity and expansion of the trans-sialidase gene family is a common feature in Trypanosoma cruzi clade members. Infect Genet Evol. 2016;37:266-74. doi: 10.1016/j.meegid.2015.11.024.

  • Stoco PH, Wagner G, Talavera-Lopez C, Gerber A, Zaha A, Thompson CE, Bartholomeu DC, Lückemeyer DD, Bahia D, Loreto E, Prestes EB, Lima FM, Rodrigues-Luiz G, Vallejo GA, Filho JF, Schenkman S, Monteiro KM, Tyler KM, de Almeida LG, Ortiz MF, Chiurillo MA, de Moraes MH, Cunha Ode L, Mendonça-Neto R, Silva R, Teixeira SM, Murta SM, Sincero TC, Mendes TA, Urmenyi TP, Silva VG, DaRocha WD, Andersson B, Romanha AJ, Steindel M, de Vasconcelos AT, Grisard EC. Genome of the avirulent human-infective trypanosome-Trypanosoma rangeli. PLOS Negl Trop Dis. 2014;8(9):e3176. doi: 10.1371/journal.pntd.0003176.

  • Peña CP, Lander N, Rodríguez E, Crisante G, Añez N, Ramírez JL, Chiurillo MA. Molecular analysis of surface glycoprotein multigene family TrGP expressed on the plasma membrane of Trypanosoma rangeli epimastigotes forms. Acta Trop. 2009;111(3):255-62. doi: 10.1016/j.actatropica.2009.05.003.

  • Moraes Barros RR, Marini MM, Antonio CR, Cortez DR, Miyake AM, Lima FM, Ruiz JC, Bartholomeu DC, Chiurillo MA, Ramirez JL, Franco da Silveira J. Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi. BMC Genomics. 2012;13:229. doi: 10.1186/1471-2164-13-229.

  • Rojas ME, Várquez P, Villarreal MV, Velandia C, Vergara L, Morán-Borges Y,  Ontiveros J, Calderón M, Chiurillo-Siervo MA, Rodríguez-Bonfante C, Aldana E, Concepción JL, Bonfante-Cabarcas R. [An entomological and seroepidemiological study of Chagas’ disease in an area in Central-Western Venezuela infested with Triatoma maculata (Erichson 1848)]. Cad Saude Publica. 2008;24(10):2323-2333. doi: 10.1590/s0102-311x2008001000013.

  • Feliciangeli MD, Chiurillo MA, Delgado O, Ruiz B. The burden of the Leishmania chagasi/infantum infection in a closed rural focus of visceral leishmaniasis in Lara state, west-central Venezuela. Trop Med Int Health. 2005;10(5)444-449. doi: 10.1111/j.1365-3156.2005.01408.x.

  • Añez-Rojas N, Peralta A, Crisante G, Rojas A, Añez N, Ramírez JL, Chiurillo MA. Trypanosoma rangeli expresses a gene of the group II trans-sialidase superfamily. Mol Biochem Parasitol. 2005;142(1) 133-136. doi: 10.1016/j.molbiopara.2005.03.012.

  • Kim D, Chiurillo MA, El-Sayeb N, Jones K, Santos MRM, Cerda PP, Andersson B, Myler P, Franco da Silveira J, Ramirez JL. Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in pseudogenes of retrotransposon hot spot and transialidase-like gene families: the origins of T. cruzi telomeres. Gene. 2005;346:153-161. doi: 10.1016/j.gene.2004.10.014.

  • Chiurillo MA, Crisante G, Rojas A, Peralta A, Dias M, Guevara P, Añez N, Ramírez JL. Detection of Trypanosoma cruzi and Trypanosoma rangeli infections with a duplex PCR assay based on telomeric sequences. Clin Diagn Lab Immunol. 2003;10(5):775-779. doi: 10.1128/cdli.10.5.775-779.2003

  • Verbisck NV, Santos MRM, Engman D, Chiurillo MA, Ramirez JL, Araya JE, Mortara RA, Franco Da Silveira J. A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Mem Inst Oswaldo Cruz. 2003;98(1): 129-133. doi: 10.1590/s0074-02762003000100016.

  • Chiurillo MA, Santos MRM, Franco Da Silveira J, Ramírez JL. A general approach for cloning and characterizing telomeres: Trypanosoma cruzi as model organism. Gene. 2002;294: 197-201. doi: 10.1016/s0378-1119(02)00768-0.

  • Chiurillo MA, Ramírez JL. Charaterization of Leishmania major Friedlin telomeric terminus. Mem Inst Oswaldo Cruz. 2002;97(3): 343-346. doi: 10.1590/s0074-02762002000300011.

  • Chiurillo MA, Peralta A, Ramírez JL. Comparative study of Trypanosoma rangeli and Trypanosoma cruzi telomeres. Mol Biochem Parasitol. 2002;120(2): 305-308. doi: 10.1016/s0166-6851(02)00005-1.

  • Chiurillo MA, Sachdeva M, Dole VS, Yepes Y, Miliani Vazquez L, Rojas A, Crisante, G, Guevara P, Añez N, Madhubala R, Luis Ramírez JL. Detection of Leishmania causing visceral leishmaniasis in the old and new world by a polymerase chain reaction assay based on telomeric sequences. Am J Trop Med Hyg. 2001;65(5):573-582. doi: 10.4269/ajtmh.2001.65.573.

  • Chiurillo MA, Beck A, Devos T, Myler PJ, Stuart K, Ramírez JL. Cloning and characterization of Leishmania donovani telomeres. Exp Parasitol. 2000;94(4):248-258. doi: 10.1006/expr.2000.4499.

  • Carmo MS, Araya JE, Ramirez MI, Boscardin S, Cano MI, Baida RP, Ruiz RC, Santos MR, Chiurillo MA, Ramírez JL, Yoshida N, Franco Da Silveira J. Organization and expression of a multigene family encoding the surface glycoproteins of Trypanosoma cruzi metacyclic trypomastigotes involved in the cell invasion. Mem Inst Oswaldo Cruz. 1999;94 (Suppl I): 169-171. doi: 10.1590/s0074-02761999000700022.

  • Santos MRM, Lorenzi H, Porcile P, do Carmo MS, Schijman A, Brandão A, Araya J E, Gomes HBM, Chiurillo MA, Ramírez JL, Degrave WM, Levin MJ, Franco Da Silveira J. Physical mapping of a large region of chromosomes XVI and XVII from the human protozoan parasite Trypanosoma cruzi encompassing the genes for two immunodominant antigens. Genome Res. 1999;9:1268-1276. doi: 10.1101/gr.9.12.1268.

  • Chiurillo MA, Cano I, Franco Da Silveira J, Ramírez JL. Organization of telomeric and subtelomeric regions of chromosomes from the protozoan parasite Trypanosoma cruzi. Mol Biochem Parasitol. 1999;100:173-183. doi: 10.1016/s0166-6851(99)00047-x.

  • Ferrari I, Lorenzi H, Santos MR, Brandariz S, Requena JM, Schijman A, Vazquez M, Da Silveira JF, Ben-Dov C, Medrano C, Guío S, López Bergami P, Cano I, Zingales B, Urmenyi TP, Rondinelli E, González A, Cortes A, López MC, Thomas MC, Alonso C, Ramírez JL, Chiurillo MA, Rangel Aldao R, Brandão A, Degrave W, Perrot V, Saumier M, Billaut A, Cohen D, Le Paslier D, Levin MJ. Towards the physical map of the Trypanosoma cruzi nuclear genome: Construction of YAC and BAC libraries of the reference clone T. cruzi CL-Brener. Mem Inst Oswaldo Cruz. 1997;9(6):843-852. doi: 10.1590/s0074-02761997000600021.

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